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Stable expression and preparation of Pool protein

Expression level: 1-4.0 g/L. Bulkpool preparation: gram-level protein (4-5 weeks). Minipool preparation: 10g-level protein (8-9 weeks).

Expression level: 1-4.0 g/L. Bulkpool produces gram-level protein (4-5 weeks), Minipool produces 10g-level protein (8-9 weeks)

Comparison Items	CHO-K1 Expression System	CHO-S and 293 Expression Systems
Source	ECACC CHO-K1 (wild type)	Thermofisher Scientific
Expression Vector	pKS001	pKS001, pCHO1.0, pCDNA3.1
Screening Method	MSX pressure screening	MSX and Puromycin pressure screening or G418 screening pressure
Transfection Method	Electroporation, efficiency over 90%	Electroporation, efficiency over 90%

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