Fully human Fab synthetic library

Fully human Fab synthetic library

Constream Biotech currently has a library capacity of 10 11 of a fully human Fab synthetic library. The ultimate goal is to establish a 100-billion-level fully human Fab synthetic library, dedicated to developing high-affinity, high-specificity candidate antibodies for pharmaceutical companies and diagnostic reagent companies.

Constream Biotech currently has a library capacity of 10 11 of a fully human Fab synthetic library. The ultimate goal is to establish a 100-billion-level fully human Fab synthetic library, dedicated to developing high-affinity, high-specificity candidate antibodies for pharmaceutical companies and diagnostic reagent companies.

Service Advantages

● Optimal germline sequences: suitable for phage display, high expression, and good druggability

● Randomization strategy: Trimer primers, diverse CDR3 lengths, and diverse amino acid distribution

● Optimal library capacity: Library capacity close to 10 11 Good diversity, reduced ineffective screening

● Widest application: Verified by dozens of targets, suitable for different types of targets, affinity reaching pM

Service Content

Serial Number

Main Steps

Content

Cycle

Delivery Results

1

Antigen Preparation

Customer provides 2mg antigen for screening and preliminary identification

 

 

2

Phage Library Screening (Fully Human Fab Synthetic Library)

1. 3-4 rounds of panning 
2. Candidate clone identification

3. ELISA ranking 
4. Sequencing and sequence analysis

4-8 weeks

Up to 40 sequences will be provided in batches (20 in the first batch, 20 in the second batch) for full antibody expression and activity verification by the customer.

3

Full Antibody Expression and Purification

The antibodies obtained from phage screening will be converted into full antibody forms (IgG1 or customer-specified full antibody forms) for expression and affinity purification.

2-3 weeks

Up to 1mg protein per sequence (concentration >1mg/ml, 1-step purification: SEC >95%, SDS-PAGE >95%)

4

Total Cycle

6-11 weeks

Service Cases

Synthetic Phage Library Display Technology Screening Cases

Name

Commercial Name

Format

Target

Company

Clinical Indication

Status

Phage Display Type

Atezolizumab

Tecentriq

Humanized IgG1

PD-L1

Roche, 
Genentech

Cancers, 
Melanoma, 
Solid Tumors, hepatocellular carcinoma, 
others

Market

Human Library 
(Fab`-zip synthetic single-framework)

Brodalumab

Kyntheum, 
Siliq

Human IgG2

IL-17RA

Valeant Pharmaceuticals; LEO Pharma

Plaque psoriasis

Market

MorphoSyś HuCAL

Faricimab

Vabysmo

Human/Humanized IgG1

VEGF-A, Ang-2

Roche

Macular degeneration

Approved

Synthetic human Fab library

Guselkumab

TREMFYA™

Human IgG1

IL23A

Morphosys (Germany); Morphosys and Janssen Biotech (USA)

Psoriatic arthritis, psoriasis, palmoplantar pustulosis, others

Approved

MorphoSyś HuCAL

Tafasitamab

Monjuvi

Humanized IgG1/2

CD19

Xencor

Non-Hodgkin’s lymphoma, chronic lymphocytic leukemia

Approved

MorphoSyś HuCAL

Tebentafusp

KIMMTRAK

Human TCR-scFv

gp100, CD3

Immunocore

Metastatic uveal melanoma

Approved

Synthetic TCR-phage library

Tralokinumab

Adbry™ (USA) 
Adtralza® (EU)

Human IgG4

IL-13

LEO Pharma

Atopic dermatitis, 
asthma

Approved

Synthetic human scFv library

Felzartamab

N/A

IgG1-lambda

CD38

HI-Bio

MM

Phase II-III

Synthetic Fab HuCAL GOLD (MorphoSyś)

Anetumab 
ravtansine

N/A

Human IgG1-lambda – DM4

MSLN

Bayer

Solid tumors, MPM

Phase II

MorphoSyś HuCAL GOLD

Carlumab

N/A

Human IgG1-kappa

CCL2

Gilead Sciences

PCa

Phase I-II

MorphoSyś HuCAL GOLD

Documents

Subscription Information

We will send you real-time information regularly!

Subscribe
Constream Bio

Email:

taoweihong@constreambio.com

Shanghai Constream biotechnology Co., Ltd.

Tel: 021-59255902

Address: 3rd Floor, Building 28, No. 10688, Beiqing Highway, Qingpu District, Shanghai

Mobile Website

Official Account

Copyright © 2024 Shanghai Constream biotechnology Co., Ltd

COOKIES

Our website uses cookies and similar technologies to personalize the advertising shown to you and to help you get the best experience on our website. For more information, see our Privacy & Cookie Policy

COOKIES

Our website uses cookies and similar technologies to personalize the advertising shown to you and to help you get the best experience on our website. For more information, see our Privacy & Cookie Policy

These cookies are necessary for basic functions such as payment. Standard cookies cannot be turned off and do not store any of your information.

These cookies collect information, such as how many people are using our site or which pages are popular, to help us improve the customer experience. Turning these cookies off will mean we can't collect information to improve your experience.

These cookies enable the website to provide enhanced functionality and personalization. They may be set by us or by third-party providers whose services we have added to our pages. If you do not allow these cookies, some or all of these services may not function properly.

These cookies help us understand what you are interested in so that we can show you relevant advertising on other websites. Turning these cookies off will mean we are unable to show you any personalized advertising.

Privacy Policy   www.300.cn