Stable expression and preparation of Pool protein
Expression levels range from 1 to 4.0 g/L; bulk pool production yields protein at the gram scale (4–5 weeks), while minipool production delivers protein at the 10-g scale (8–9 weeks).
Expression levels range from 1 to 4.0 g/L; bulk pool production yields protein at the gram scale (4–5 weeks), while minipool production delivers protein at the 10-g scale (8–9 weeks).
Comparison Items | CHO-K1 expression system | CHO-S and the 293 expression system |
Source | ECACC CHO-K1 (wild-type) | Thermofisher Scientific |
Expression vector | pKS001 | pKS001, pCHO1.0, pCDNA3.1 |
Filtering method | MSX Pressure Screening | MSX and puromycin selection pressure or G418 selection pressure |
Transfection method | Electrical conversion efficiency exceeds 90%. | Electrical conversion efficiency exceeds 90%. |
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